Following the identification of a large number of dysregulated miRNAs in plasma samples from DMD patients and GRMD dogs, we have now demonstrated the dysregulation of many of these miRNAs, including Dlk1-Dio3 miRNAs, within the skeletal muscle, using the GRMD dog as a model of DMD. The gene discussed is DLK1; the disease is Duchenne muscular dystrophy.