VIC were cultured for 2, 7, 14 and 21 days in C, HG, OM and HGOM and the protein expression levels of myofibroblast differentiation marker, α-SMA and of osteogenic markers, namely Runx2, ALP and BSP were determined by Western blot assay (Figure 2). The gene discussed is IBSP; the disease is ocular melanoma.