Throughout the tumor areas of the Py230 orthotopic breast tumor model, the walls of neovessels were strongly positive (2–3+) for anxA1 staining by endpoint day 44; however, the signal was localized to the vascular smooth-muscle cells, and the subjacent endothelial cells were negative for anxA1 (Fig 3B). Here, ANXA1 is linked to breast neoplasm.