In summary, our results revealed that circSLAMF6 knockdown played an anti-cancer role by inhibiting cell glycolysis, migration, and invasion through sponging miR-204-5p and regulating MYH9 in GC under hypoxia, providing a novel insight into the pivotal role of circRNA-miRNA-mRNA functional network in GC development. This evidence concerns the gene MYH9 and cancer.