Meanwhile, the results of LY294002 were in accord with CQ in both breast cancer cells (Fig. 4c, d). Caspase-3/7 analysis also revealed that inhibiting autophagy by CQ or LY294002 remarkably reduced T-DM1-induced Caspase-3/7 activation level (Fig. 4e, f). The gene discussed is CASP3; the disease is breast cancer.