In addition, administration of constitutive androstane receptor (CAR) ligand (normally used to improve insulin sensitivity in the context of obesity) to dams fed a high-fat diet during gestation ameliorates the changes in acetyl H3K9 and dimethyl H3K9 that are associated with the adiponectin and leptin gene promoters and normalises circulating concentrations in the offspring [219]. This evidence concerns the gene NR1I3 and obesity due to melanocortin 4 receptor deficiency.