ACTA2 and cryohydrocytosis: Our study done in 2014 revealed strong association of GFAP with the gold standard immunohistochemical marker, ACTA2 suggesting that GFAP could be a useful indicator of early HSCs activation in CHC patients.10 The GFAP positive hepatic cells may be antecedents of the HSCs detected by ACTA2 or they may denote a diverse subpopulation.11,12