In the current study, we have shown that the three Illumina 5′-CpG sites associated with the analysed ddPCR amplicon were strongly methylated and that PLA2R1 expression was completely or notably suppressed in six childhood B-ALL and eight T-ALL cell lines, including the investigated Jurkat cell line (Supplementary Table 2). Here, PLA2R1 is linked to acute lymphoblastic leukemia.