To search for novel cell surface markers on EMT-phenotypic-circulating tumor cells, in the previous study, we generated a panel of monoclonal antibodies (MAbs) against surface molecules on TGF-β1-treated A549 human non-small cell lung carcinoma cells (NSCLC) because TGF-β1-treated A549 cells represent EMT-phenotypic cells [20–22]. The gene discussed is TGFB1; the disease is non-small cell lung carcinoma.