Through bioinformatics analysis, gene expression patterns and dual luciferase reporter gene assays, we found that miR-550a-3-5p directly targeted YAP which emerged to exhibit great roles in cancer immunity, and further regulated M2 macrophages polarization and migration, invasion and EMT of HPV-positive OSCC cells via YAP/CCL2 signaling. This evidence concerns the gene CCL2 and cancer.