As figures 2 to 5 show, 72 h incubation of BT-20, BT-549, MB- 468, and MCF-7 cell lines with IRAK inhibitor and MTX significantly increased the annexin-V and annexin-V/7AAD positive cells, suggesting an apoptotic effect of IRAK on all tested BC cell lines. Here, ANXA5 is linked to breast cancer.