PMP22 and Charcot-Marie-Tooth disease: To identify genetic cause of the disease, screening for the prevalent CMT-causing variations i.e. peripheral myelin protein 22 (PMP22; OMIM 601097), duplication and point mutations in myelin protein zero (MPZ; OMIM 159440), and mitofusin 2 (MFN2; OMIM 608507) genes, had been performed using MLPA and PCR-based Sanger sequencing, respectively.