ANA was ordered in 100 patients. A positive ANA was noted in 52 subjects (52.0%). From these 52 patients, 26 had a positive MAA (26.0%). Of those patients with a positive ANA, five had an ANA titer of 1:320 or less. There were 17 patients with a negative ANA who ultimately had a positive MAA on the extended myositis antibody panel (17.0%). Here, BTG3 is linked to myositis disease.