AKT1 and neoplasm: Protein analysis by WB also confirmed these findings that tumors in TG livers expressed a higher level of PDGFRα, phosphor-Akt (Thr308), cyclin D1 and c-myc, but lower level of MET and EGFR, although protein contamination by non-tumorous tissue might be not fully avoid when harvested because of the small size of each tumor (Figure 6C).