Because bioinformatics analysis suggested that XIST may be a target gene regulating miR-204-5p, qRT-PCR verified that the expression of XIST was negatively correlated with miR-204-5p expression in glioma tissues, and we further investigated whether miR-204-5p is negatively regulated by XIST in glioma cells. The gene discussed is XIST; the disease is central nervous system cancer.