Furthermore, PDK4 protein was observed in both the nucleus and cytoplasm of 7402 and 7404 cells based on an immunofluorescence assay (Fig. 1F), and PDK4 protein was also detected in the nucleus and cytoplasm of cancer cells contained in HCC clinical tissue specimens based on IHC (Fig. 1A). The gene discussed is PDK4; the disease is hepatocellular carcinoma.