ITGA6 and hypertrophic cardiomyopathy: In P4, the different proteins were enriched in microRNA in cancer, small cell lung cancer, hypertrophic cardiomyopathy and dilated cardiomyopathy due to the proteins such as TIMP3 (Metalloproteinase inhibitor 3), ITGA6 (Integrin alpha-6) and TPMs (Tropomyosins) were affected by culturing from 24 h to 48 h (P4N24 vs. P4N48) and freeze–thawing for culturing 24 h (P4N24 vs. P4C24), and these gene were clustered in pathway of those disease.