HMGB1 and neoplasm: Subsequently, qRT-PCR analysis revealed that sh-NNT-AS1 transfection decreased NNT-AS1 expression in vivo, besides that, the expression of miR-186 was increased, while HMGB1 mRNA or protein expression was reduced in tumor tissues derived from sh-NNT-AS1-transfected CC cells (Fig. 8c, d).