The results of qRT-PCR, western blot, and immunohistochemistry showed that HK-2 expression was decreased in SALL4 knockdown group compared to control group in both models (Fig. 6b, c, d, f and g), suggesting that SALL4 knockdown decreases HK-2 expression, which in turn suppresses glycolysis in gastric cancer cells and disturbs cancer progression. Here, HK2 is linked to gastric cancer.