ESCC related genes were mostly found with amplified gene copy number, i.e. TP53, SOX2, EGFR, MYC, CCND1, CDKN2A, NOTCH1, BRCA1, NFE2L2, etc. While only TP53 and NFE2L2 were found in SNAs (Fig. 5). The gene discussed is NOTCH1; the disease is esophageal squamous cell carcinoma.