PNPLA3 and fatty liver disease: Next, we observed sporadic fatty liver phenotypes in Q140K+/+ males, but not WT animals (Fig. 4b and Supplementary Fig. 5) and found in the same males altered gene expression in markers of fatty liver disease (Pnpla2, p = 0.012; Pnpla3, p = 0.050; and G6pd, p = 0.032, Student’s t-tests; Fig. 4c and Supplementary Fig. 5).