To confirm whether RUNX1-IT1 plays a biological role in PC via the transcription factor RUNX1, we first established stable PANC-1 and SW1990 cells ectopically overexpressing RUNX1-IT1 via a lentiviral infection system (Additional file 9: Supplementary Fig. S3g). This evidence concerns the gene RUNX1 and pachyonychia congenita.