To further investigate whether RUNX1 has similar biological functions in PC, we constructed stable RUNX1 knockdown PANC-1 and SW1990 cells via a lentiviral infection system (Additional file 9: Supplementary Fig. S3e, f), and EdU and transwell assays indicated that knockdown of RUNX1 inhibited PC cell proliferation, migration and invasion (Fig. 3i–l). Here, RUNX1 is linked to pachyonychia congenita.