PKM and hepatocellular carcinoma: PKM2 low cells behaved in a similar manner to the responder-like cells in 2D culture with both cell lines demonstrating a statistically significant decrease in viability upon doxorubicin treatment alone (Viability fold change: HCC 3796: 0.64, p < 0.01; HCC 4006: 0.67, p = 0.03) (Fig. 6b, Left panels).