Liu et al. studied the mechanism of action in detail and reported that the disulfiram/copper mixture inhibits Bcl2 and induced Bax protein expression in all GBM cell lines, induces ROS activity, activates the apoptosis JNK pathway, causes ROS-dependent activation of the JNK and p38 pathways, and inhibits NFkB and ALDH activity [81]. This evidence concerns the gene MAPK8 and glioblastoma.