CD80 and neoplasm: At the present, only a limited number of intracellular or surface markers exist for the assessment of M1 activated macrophages (e.g., iNOS, CD68, CD80) [15,44], however, as recent single-cell profiling approaches have demonstrated, canonical M1 and M2 macrophage markers are co-expressed on many subsets of tumor-associated macrophages [45].