Our results confirmed the intracellular location of HtrA2 (Figure 3) and showed that, in CRC tissue, the HtrA2 protein level was significantly decreased when compared to the unchanged colorectal mucosa (Figure 1B), especially in tumors from patients with metastatic disease (combined stages III and IV) (Figure 2B). The gene discussed is HTRA2; the disease is metastatic neoplasm.