VSV-GFP infection was evident in treatments with supernatant from nsp15 and D265A mutant transfected cells, and conversely VSV-GFP infection was obviously inhibited in treatments with supernatant from cells transfected with the remaining mutants (H226A, H241A, and K282A) or empty vector, demonstrating that nsp15 was an antagonistic protein in IFN production (Figure 6B). The gene discussed is IFNA1; the disease is infection.