The biggest fold differences in antibody binding between anti-CCP-positive RA patients and healthy donors were observed for spots containing the calcium-regulated heat-stable protein 1 (35-fold compared with healthy donor plasma), testis-specific Y-encoded protein 3 (28-fold), and Cytosolic Fe-S cluster assembly factor NUBP2 (28-fold) (Figure 2 and Table S2). This evidence concerns the gene CARHSP1 and rheumatoid arthritis.