However, transduction with the ABL sgRNA_1 or ABL sgRNA_2 virus led to multiple gene disruptions at the predicted cleavage sites (red arrowhead) of both protospacers, with more pronounced effects in ABL sgRNA_2-transduced CML cells than ABL sgRNA_1-transduced CML cells (Figure 5F,G). The gene discussed is ABL1; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.