Experiments using this approach show that male-specific depletion of Msl2 leads to similar effects to the ones observed when inducing CIN by knocking down elements of the SAC, namely, ROS production, JNK activation as well as apoptosis and tumour formation when cells are apoptosis-resistant [22,23]. This evidence concerns the gene MAPK8 and cervical squamous intraepithelial neoplasia.