High TRPM4 protein intensity was associated with increased number of tumor buds and infiltrative growth pattern in colorectal cancer patient cases, and late-stage metastatic colorectal cancer cell lines had higher TRPM4 protein expression [24]; (3) TRPM4 transcript expression was positively associated with EMT genes of adhesion molecules or extracellular matrix origin including collagens (e. g. COL1A2, COL5A1, COL6A3) and extracellular matrix 1 (ECM1) in our GSEA results. This evidence concerns the gene COL6A3 and colorectal cancer.