Following PARP1 immunoprecipitation, immunoblotting with antibodies specific for PAR revealed that PARP1 autoPARylation was significantly increased after pharmacological inhibition of PARG with PDD-00017273 in Hep3B cells (Figure 4C-D), indicating that PARG inhibition increases PARP1 autoPARylation in HCC cells. This evidence concerns the gene PARP1 and hepatocellular carcinoma.