To monitor the dynamic expression of STIM1 during HCC cell invasion and metastasis, we established EMT models of SMMC7721, HepG2, Hep3B, and BEL-7404 cells via treatment with transforming growth factor beta 1 (TGF-β1) or under hypoxic condition. This evidence concerns the gene TGFB1 and hepatocellular carcinoma.