To unequivocally prove that MGAT3 is the enzyme responsible for CD82 glycosylation in ovarian cancer cells, we treated protein lysates from CD82 and N157Q mutant cells with recombinant PNGase F to remove the glycosylation moiety and subsequently subjected these lysates to treatment with recombinant MGAT3. The gene discussed is MGAT3; the disease is ovarian cancer.