MYCN and neuroblastoma: To further investigate the potential link between the level of MYCN expression and cell response to glutamine deprivation, we utilized as a model the SH-EP/TET21N human neuroblastoma cell line, in which MYCN expression can be repressed by doxycycline, and two non-MYCN-amplified neuroblastoma cell lines, SH-SY5Y and SK-N-AS, which were stably overexpressing MYCN (Figure S1B).