MYCN and neuroblastoma: To examine the importance of Myc member expression to glutamine deprivation conditions, four cell lines representing high-risk neuroblastoma disease, two with high c-Myc expression (SK-N-AS and SH-SY5Y) and two with MYCN amplification (BE(2)-C and Kelly) (Figure 1A), were cultured in glutamine-free media and their proliferation rate and viability were assessed periodically.