Intriguingly, metformin treatment in 3xTg-AD mice reduced Mgll mRNA expression, showing a non-significant change relative to Non-Tg/control mice (Figure 4G, two-way ANOVA: Genotype x treatment F (1, 39) = 0.1481, P = 0.70; Genotype F (1, 39) = 18.63, P = 0.0001; Treatment F (1, 39) = 0.6555, P = 0.42, n = 43, Tukey's post-hoc test, ** P < 0.01). Here, MGLL is linked to Alzheimer disease.