We observed that metformin treatment successfully reversed the increased Mgll expression in 3xTg-AD mice back to the basal level (Non-Tg/control) (Figure 4A-B, two-way ANOVA: Genotype x treatment F (1, 22) = 7.333, P = 0.0128; Genotype F (1, 22) = 3.423, P = 0.0778; Treatment F (1, 22) = 5.151, P = 0.0334, n = 6-7, Tukey's post-hoc test, *P<0.05, **P<0.01). Here, MGLL is linked to Alzheimer disease.