Then, we identified the direct binding of SIRT6 at the CYP24A1 gene locus in cells with not only endogenous SIRT6 but also overexpressed HA-SIRT6 (Figure S9F), suggesting that CYP24A1 is a newly identified direct downstream target gene of SIRT6 in CRC cells and that MDL-811 inhibits CYP24A1 gene transcription by decreasing the levels of H3K9Ac and H3K18Ac. This evidence concerns the gene CYP24A1 and colorectal carcinoma.