CYP24A1 and colorectal carcinoma: We assessed the proliferation of HCT116 cells with CYP24A1 overexpression (Figure S11) and observed that compared with the control groups, CYP24A1-overexpressing cells were resistant to MDL-811 treatment (Figure 5C), indicating that CYP24A1 downregulation could mediate the decrease in CRC cell proliferation induced by MDL-811.