GSEA was conducted to investigate the relationship between JMJD2B and autophagy-related gene signatures in two independent colon cancer datasets (TCGA (458 colon cancer cases) and GSE39582 (443 colon cancer cases)) (Figure 3A), and a positive relation between JMJD2B and autophagy-related gene signatures was demonstrated, similar our previous gene microarrays from JMJD2B-silenced cells (Figure 3A) 3. This evidence concerns the gene KDM4B and colonic neoplasm.