Our study demonstrates that PTBP1 binds to the adjacent polypyrimidine sequence of Axl exon 10 and competitively inhibits U2AF2 binding, thereby inhibiting the assembly of splicing complex A. We also showed that skipping Axl exon 10 generates the Axl-S isoform, which ultimately promotes the metastasis process of liver cancer (Figure 7). Here, AXL is linked to liver cancer.