PNO1 and neoplasm: To further investigate the role of PNO1 in the tumor progression of LUAD, we selected two wild-type LUAD cell lines (A549 and NCI-H1299) to construct cell lines with stable PNO1 downregulation (sh-PNO1) and PNO1 upregulation (PNO1) via transfection with lentiviral plasmids.