In this study, by using database exploration of The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) followed by immunohistochemical (IHC) staining validation of LUAD and lung squamous cell carcinoma (SCLC) patient samples, we confirmed that high expression of PNO1 was intently related to the poor prognosis of LUAD. This evidence concerns the gene PNO1 and small cell lung carcinoma.