Using specific-pathogen-free male Sprague Dawley rats fed with high fat diet as a NASH model, Yin and colleagues found that high dose genistein decreased NASH activity score, hepatic triglyceride levels, insulin resistance, plasma endotoxin levels and hepatic toll-like receptor-4 expression [10]. The gene discussed is TLR4; the disease is metabolic dysfunction-associated steatohepatitis.