TRPV4 and cyst: Moreover, it was shown in isolated collecting duct (CD)-derived cyst monolayers are dilated (which are dilated) that the activity of TRPV4 was impaired, it was abnormally located at subcellular levels and that its glycosylation was disrupted; hence, the levels of [Ca2+]i and the signaling pathways associated to this ion were also diminished.