Surprisingly, we found no significant differences in the prevalence of DN and DR, and DN-related markers across quartiles of circulating Nrg4 and circulating Nrg4 was also not associated with the prevalence of DN and DR, and DN-related markers, suggesting that circulating Nrg4 levels may not involve in the pathogenesis of diabetic microvascular complications other than DPN, and the three diabetic microvascular complications might have other different pathogenetic mechanisms. Here, NRG4 is linked to liver dysplastic nodule.