CD4 and neoplasm: In order to illustrate the underlying mechanisms of Bcl6 in regulating Treg cell response during tumorigenesis, we sorted antigen experienced Treg, Th1 and Tfh cells as well as naive Treg cells from WT and KO mice at 16 days after tumor implantation to extract total RNA and conduct an array of quantitative real-time PCR (RT-qPCR) to profile the transcriptional signatures of the indicated subsets of CD4 T cells.