To investigate whether dLNs derived Tfr cells were involved in tumor control, we set up BM chimera mice by mixing bone marrow cells derived from Foxp3DTR mice (50%) and Cxcr5–/– mice (50%) and injecting the mixtures into sub-lethally irradiated CD4–/– recipients (Figure 3A). This evidence concerns the gene CXCR5 and neoplasm.