In vivo studies demonstrated the potent anti-viral, and anti-neuroinflammatory effect of NBE, where NBE decreases viral replication, viral transcription, viral N protein synthesis, expression of inflammatory cytokines IL-6 and IL-10, viral-induced acute stage hepatitis, encephalomyelitis, and chronic stage neuroinflammatory demyelination (Supplementary Figure S3). This evidence concerns the gene IL6 and hepatitis A virus infection.