EB leakage might have occurred because of BBB disruption and also because of ischemia-induced opening of connexin hemichannels in neurovascular unit constituent cells, making them permeable to EB dye (Orellana et al., 2013); however, TRPV4 has potential as a therapeutic target for post-ischemic brain injury from the perspective of BBB protection. Here, TRPV4 is linked to ischemia.