Therefore, we next applied our imaging and analysis strategy to explore the potential for fragmented mitochondria in breast cancer cells that lack either the Rho-like family of Rho-GTPases RhoA or RhoC via CRISPR/Cas9 knockout (WT, RhoA KO, RhoC KO) (Supplementary Fig. 8A). The gene discussed is RHOC; the disease is breast carcinoma.