We did not observe NTD in Wnt1-cre mediated Dot1l-cKO embryos at any time point during neurogenesis (Figure S1C), notwithstanding the indications that altered levels of H3K79me2 associate with closure defects of the neural tube in humans [43], and pharmacological inhibition of DOT1L increased occurrence of NTD in chicken (Figure S1D, E). Here, DOT1L is linked to neural tube defect.