ALK and cancer: To compare the performance parameters (i.e., precision, recall, and F-measure) of Fcirc with those of other methods, we took advantage of RNA-Seq data from spike-in experiments, which included 9 synthetic cancer-associated fusion genes (EWSR1-ATF1, TMPRSS2-ETV1, EWSR1-FLI1, NTRK3-ETV6, CD74-ROS1, HOOK3-RET, EML4-ALK, AKAP9-BRAF, and BRD4-NUTM1) [21].