GPT and cancer: Finally, we assessed whether methylation of the TERT promoter, either WT or mutant, would silence expression in a luciferase construct in multiple cell models, including LNCaP (TERT WT cancer cell line), SNB-19 (TERT mutant cancer cell line), U2OS (telomerase-negative, ALT-positive cancer cell line), and HEK293T (immortalized cell line).